Understanding dynamics of Rhizophagus irregularis ontogenesis in axenically developed coculture through basic and advanced microscopic techniques.
Identifieur interne : 000287 ( Main/Exploration ); précédent : 000286; suivant : 000288Understanding dynamics of Rhizophagus irregularis ontogenesis in axenically developed coculture through basic and advanced microscopic techniques.
Auteurs : Shikha Chaudhary [Inde] ; Priyanka Gupta [Inde] ; Shivani Srivastava [Inde] ; Alok Adholeya [Inde]Source :
- Journal of basic microbiology [ 1521-4028 ] ; 2019.
Descripteurs français
- KwdFr :
- MESH :
- croissance et développement : Glomeromycota, Hyphae, Mycorhizes.
- microbiologie : Racines de plante.
- instrumentation : Culture axénique, Microscopie, Ontologies biologiques.
English descriptors
- KwdEn :
- MESH :
- growth & development : Glomeromycota, Hyphae, Mycorrhizae.
- instrumentation : Microscopy.
- microbiology : Plant Roots.
- Axenic Culture, Biological Ontologies.
Abstract
Detailed information on structural changes that occur during ontogenesis of Rhizophagus irregularis in axenically developed coculture is limited. Our study aims to investigate the series of events that occur during mycorrhizal ontogenesis under axenic condition through basic and advanced microscopic techniques followed by comparison among these to identify the suitable technique for rapid and detailed analysis of mycorrhizal structures. Three stages were identified in mycorrhizal ontogenesis from initiation (preinfection stage of hyphae; its branching, infection and appressoria formation; epidermal opening; and hyphal entry), progression (arbuscular development; hyphal coils and vesicles) to maturity (extraradical spores). Scanning electron microscopy was found to be an efficient tool for studying spatial three-dimensional progression. Adding to the advantages of advanced microscopy, potential of autofluorescence to explore the stages of symbiosis nondestructively was also established. We also report imaging of ultrathin sections by bright field microscopy to provide finer details at subcellular interface. Owing to the merits of nondestructive sampling, ease of sample preparation, autofluorescence (no dye required), no use of toxic chemicals, rapid analysis and in depth characterization confocal laser scanning microscopy was identified as the most preferred technique. The method thus developed can be used for detailed structural inquisition of mycorrhizal symbiosis both in in planta and in an in vitro system.
DOI: 10.1002/jobm.201900138
PubMed: 31074496
Affiliations:
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Haryana</wicri:regionArea><wicri:noRegion>Haryana</wicri:noRegion></affiliation></author><author><name sortKey="Gupta, Priyanka" sort="Gupta, Priyanka" uniqKey="Gupta P" first="Priyanka" last="Gupta">Priyanka Gupta</name><affiliation wicri:level="1"><nlm:affiliation>TERI-Deakin Nanobiotechnology Centre, TERI Gram, The Energy and Resources Institute (TERI), Gwal Pahari, Gurugram, Haryana, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>TERI-Deakin Nanobiotechnology Centre, TERI Gram, The Energy and Resources Institute (TERI), Gwal Pahari, Gurugram, Haryana</wicri:regionArea><wicri:noRegion>Haryana</wicri:noRegion></affiliation></author><author><name sortKey="Srivastava, Shivani" sort="Srivastava, Shivani" uniqKey="Srivastava S" first="Shivani" last="Srivastava">Shivani Srivastava</name><affiliation wicri:level="1"><nlm:affiliation>TERI-Deakin Nanobiotechnology Centre, TERI Gram, The Energy and Resources Institute (TERI), Gwal Pahari, Gurugram, Haryana, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>TERI-Deakin Nanobiotechnology Centre, TERI Gram, The Energy and Resources Institute (TERI), Gwal Pahari, Gurugram, Haryana</wicri:regionArea><wicri:noRegion>Haryana</wicri:noRegion></affiliation></author><author><name sortKey="Adholeya, Alok" sort="Adholeya, Alok" uniqKey="Adholeya A" first="Alok" last="Adholeya">Alok Adholeya</name><affiliation wicri:level="1"><nlm:affiliation>TERI-Deakin Nanobiotechnology Centre, TERI Gram, The Energy and Resources Institute (TERI), Gwal Pahari, Gurugram, Haryana, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>TERI-Deakin Nanobiotechnology Centre, TERI Gram, The Energy and Resources Institute (TERI), Gwal Pahari, Gurugram, Haryana</wicri:regionArea><wicri:noRegion>Haryana</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of basic microbiology</title><idno type="eISSN">1521-4028</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Axenic Culture (MeSH)</term><term>Biological Ontologies (MeSH)</term><term>Glomeromycota (growth & development)</term><term>Hyphae (growth & development)</term><term>Microscopy (instrumentation)</term><term>Mycorrhizae (growth & development)</term><term>Plant Roots (microbiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Culture axénique (MeSH)</term><term>Glomeromycota (croissance et développement)</term><term>Hyphae (croissance et développement)</term><term>Microscopie (instrumentation)</term><term>Mycorhizes (croissance et développement)</term><term>Ontologies biologiques (MeSH)</term><term>Racines de plante (microbiologie)</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Glomeromycota</term><term>Hyphae</term><term>Mycorhizes</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Glomeromycota</term><term>Hyphae</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Microscopy</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Racines de plante</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Roots</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Axenic Culture</term><term>Biological Ontologies</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="fr"><term>Culture axénique</term><term>Microscopie</term><term>Ontologies biologiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Detailed information on structural changes that occur during ontogenesis of Rhizophagus irregularis in axenically developed coculture is limited. Our study aims to investigate the series of events that occur during mycorrhizal ontogenesis under axenic condition through basic and advanced microscopic techniques followed by comparison among these to identify the suitable technique for rapid and detailed analysis of mycorrhizal structures. Three stages were identified in mycorrhizal ontogenesis from initiation (preinfection stage of hyphae; its branching, infection and appressoria formation; epidermal opening; and hyphal entry), progression (arbuscular development; hyphal coils and vesicles) to maturity (extraradical spores). Scanning electron microscopy was found to be an efficient tool for studying spatial three-dimensional progression. Adding to the advantages of advanced microscopy, potential of autofluorescence to explore the stages of symbiosis nondestructively was also established. We also report imaging of ultrathin sections by bright field microscopy to provide finer details at subcellular interface. Owing to the merits of nondestructive sampling, ease of sample preparation, autofluorescence (no dye required), no use of toxic chemicals, rapid analysis and in depth characterization confocal laser scanning microscopy was identified as the most preferred technique. The method thus developed can be used for detailed structural inquisition of mycorrhizal symbiosis both in in planta and in an in vitro system.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31074496</PMID><DateCompleted><Year>2019</Year><Month>08</Month><Day>09</Day></DateCompleted><DateRevised><Year>2019</Year><Month>08</Month><Day>09</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1521-4028</ISSN><JournalIssue CitedMedium="Internet"><Volume>59</Volume><Issue>8</Issue><PubDate><Year>2019</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Journal of basic microbiology</Title><ISOAbbreviation>J Basic Microbiol</ISOAbbreviation></Journal><ArticleTitle>Understanding dynamics of Rhizophagus irregularis ontogenesis in axenically developed coculture through basic and advanced microscopic techniques.</ArticleTitle><Pagination><MedlinePgn>767-774</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/jobm.201900138</ELocationID><Abstract><AbstractText>Detailed information on structural changes that occur during ontogenesis of Rhizophagus irregularis in axenically developed coculture is limited. Our study aims to investigate the series of events that occur during mycorrhizal ontogenesis under axenic condition through basic and advanced microscopic techniques followed by comparison among these to identify the suitable technique for rapid and detailed analysis of mycorrhizal structures. Three stages were identified in mycorrhizal ontogenesis from initiation (preinfection stage of hyphae; its branching, infection and appressoria formation; epidermal opening; and hyphal entry), progression (arbuscular development; hyphal coils and vesicles) to maturity (extraradical spores). Scanning electron microscopy was found to be an efficient tool for studying spatial three-dimensional progression. Adding to the advantages of advanced microscopy, potential of autofluorescence to explore the stages of symbiosis nondestructively was also established. We also report imaging of ultrathin sections by bright field microscopy to provide finer details at subcellular interface. Owing to the merits of nondestructive sampling, ease of sample preparation, autofluorescence (no dye required), no use of toxic chemicals, rapid analysis and in depth characterization confocal laser scanning microscopy was identified as the most preferred technique. The method thus developed can be used for detailed structural inquisition of mycorrhizal symbiosis both in in planta and in an in vitro system.</AbstractText><CopyrightInformation>© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chaudhary</LastName><ForeName>Shikha</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>TERI-Deakin Nanobiotechnology Centre, TERI Gram, The Energy and Resources Institute (TERI), Gwal Pahari, Gurugram, Haryana, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gupta</LastName><ForeName>Priyanka</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>TERI-Deakin Nanobiotechnology Centre, TERI Gram, The Energy and Resources Institute (TERI), Gwal Pahari, Gurugram, Haryana, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Srivastava</LastName><ForeName>Shivani</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>TERI-Deakin Nanobiotechnology Centre, TERI Gram, The Energy and Resources Institute (TERI), Gwal Pahari, Gurugram, Haryana, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Adholeya</LastName><ForeName>Alok</ForeName><Initials>A</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-8116-8045</Identifier><AffiliationInfo><Affiliation>TERI-Deakin Nanobiotechnology Centre, TERI Gram, The Energy and Resources Institute (TERI), Gwal Pahari, Gurugram, Haryana, India.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><Agency>TERI-Deakin Nanobiotechnology Centre</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>06</Month><Day>18</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>J Basic Microbiol</MedlineTA><NlmUniqueID>8503885</NlmUniqueID><ISSNLinking>0233-111X</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D061245" MajorTopicYN="N">Axenic Culture</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D064229" MajorTopicYN="N">Biological Ontologies</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055137" MajorTopicYN="N">Glomeromycota</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D025301" MajorTopicYN="N">Hyphae</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008853" MajorTopicYN="Y">Microscopy</DescriptorName><QualifierName UI="Q000295" MajorTopicYN="N">instrumentation</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">arbuscule</Keyword><Keyword MajorTopicYN="N">autofluorescence</Keyword><Keyword MajorTopicYN="N">life cycle</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>03</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>04</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>05</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>5</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>8</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>5</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31074496</ArticleId><ArticleId IdType="doi">10.1002/jobm.201900138</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Inde</li></country></list><tree><country name="Inde"><noRegion><name sortKey="Chaudhary, Shikha" sort="Chaudhary, Shikha" uniqKey="Chaudhary S" first="Shikha" last="Chaudhary">Shikha Chaudhary</name></noRegion><name sortKey="Adholeya, Alok" sort="Adholeya, Alok" uniqKey="Adholeya A" first="Alok" last="Adholeya">Alok Adholeya</name><name sortKey="Gupta, Priyanka" sort="Gupta, Priyanka" uniqKey="Gupta P" first="Priyanka" last="Gupta">Priyanka Gupta</name><name sortKey="Srivastava, Shivani" sort="Srivastava, Shivani" uniqKey="Srivastava S" first="Shivani" last="Srivastava">Shivani Srivastava</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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